OSI-930
作者：齊岳
發(fā)布時間：2023-08-23
09:37:58
點(diǎn)擊數(shù)：
產(chǎn)品名稱：OSI-930
產(chǎn)品描述：
| 產(chǎn)品描述 | OSI-930, an orally active inhibitor of c-Kit and the vascular endothelial growth factor receptor-2 (VEGFR-2), targets cancer cell proliferation and blood vessel growth (angiogenesis) in tumors. |
| 靶點(diǎn)活性 | Flt1:8 nM, CSF-1R:15 nM, C-Raf:41 nM, Lck:22 nM, KDR:9 nM |
| 體外活性 | 在HMC-1, NCI-SNU-5, COLO-205和U251移植瘤模型中,口服OSI-930（200 mg/kg）能夠夠抑制腫瘤細(xì)胞增殖. |
| 體內(nèi)活性 | 在HMC-1 細(xì)胞系中（IC50=14 nM）,OSI-930抑制細(xì)胞增殖,誘導(dǎo)細(xì)胞凋亡。OSI-930能夠抑制多種重組受體酪氨酸激酶,Kit（IC50=80 nM）, KDR（IC50=9 nM）, Flt（ IC50=8 nM）, CSF-1R（IC50=15 nM）, c-Raf （IC50=41 nM）和Lck（IC50=22 nM）。 |
| 激酶實(shí)驗 | Protein kinase assays : Protein kinase assays are either done in-house by ELISA-based assay methods (Kit, KDR, PDGFRα, and PDGFRβ) or by a radiometric method. In-house ELISA assays used poly(Glu:Tyr) as the substrate bound to the surface of 96-well assay plates; phosphorylation is then detected using an antiphosphotyrosine antibody conjugated to HRP. The bound antibody is then quantitated using ABTS as the peroxidase substrate by measuring the absorbance at 405/490 nm. All assays uses purified recombinant kinase catalytic domains that are either expressed in insect cells or in bacteria. The Kit and EGFR protein used for in-house assays are prepared internally; other enzymes are obtained. Recombinant Kit protein is expressed as an NH2-terminal glutathione S-transferase fusion protein in insect cells and is initially purified as a nonphosphorylated (nonactivated) enzyme with a relatively high Km for ATP (400 μM). In some assays, an activated (tyrosine phosphorylated) form of the enzyme is prepared by incubation with 1 mM ATP for 1 hour at 30 °C. The phosphorylated protein is then passed through a desalting column to remove the majority of the ATP and stored at ?80 °C in buffer containing 50% glycerol. The resultant preparation has a considerably higher specific activity and a lower Km for ATP (25 μM) than the initial nonphosphorylated preparation. The inhibition of Kit autophosphorylation by OSI-930 is assayed by incubation of the nonphosphorylated enzyme at 30 °C in the presence of 200 μM ATP and various concentrations of OSI-930. The reaction is stopped by removal of aliquots into SDS-PAGE sample buffer followed by heating to 100 °C for 5 minutes. The degree of phosphorylation of Kit is then determined by immunoblotting for both total Kit and phosphorylated Kit. |
| 細(xì)胞實(shí)驗 | For assays of cell proliferation and apoptosis, cells are seeded into 96-well plates and incubated for 2 to 3 days in the presence of OSI-930 at various concentrations. Inhibition of cell growth is determined by luminescent quantitation of the intracellular ATP content using CellTiterGlo. Induction of caspase-dependent apoptosis by OSI-930 is quantitated by an enzymatic caspase 3/7 assay. Inhibition of angiogenesis by OSI-930 is monitored using the rat aortic ring endothelial sprout outgrowth assay. Sections of aorta are prepared from CO2-euthanized male rats and cultured in vitro in a collagen matrix in the presence or absence of OSI-930. The collagen matrix is prepared from type 1 rat tail collagen solubilized in 0.1% acetic acid at 3 mg/mL, which is combined with 0.125 volume collagen buffer (0.05 N NaOH, 200 mM HEPES, 260 mM NaHCO3), 0.125 volume of medium 199, 0.0125 volume of 1 M NaOH, and 1% GlutaMax. Aortic rings are embedded in 0.4 mL of this matrix in six-well plates, to which 0.5 mL endothelial basal medium and the appropriate amount of OSI-930 is added; the rings are then incubated for 10 days and the resultant angiogenic sprout outgrowth is digitally quantitated from images by measurement of the sprout-containing area within a series of concentric rings around the aortic tissue area.(Only for Reference) |
| 別名 | OSI 930, 噻爾非尼 |
| 分子量 | 443.44 |
| 分子式 | C22H16F3N3O2S |
| CAS No. | 728033-96-3 |
存儲
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 82 mg/mL (184.9 mM)
Ethanol: 3 mg/mL (6.76 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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